Status:

UNKNOWN

Vaccine Therapy in Treating Patients With Stage IV Melanoma

Lead Sponsor:

Dermatologische Klinik MIT Poliklinik-Universitaetsklinikum Erlangen

Conditions:

Melanoma (Skin)

Eligibility:

All Genders

18+ years

Phase:

PHASE1

PHASE2

Brief Summary

RATIONALE: Vaccines made from a person's tumor cells and white blood cells may help the body build an effective immune response to kill tumor cells. PURPOSE: This phase I/II trial is studying the sid...

Detailed Description

OBJECTIVES: * Determine the safety and tolerability of vaccine therapy comprising autologous dendritic cells (DC) transfected with autologous polymerase chain reaction-amplified tumor RNA in patients...

Eligibility Criteria

Inclusion

  • DISEASE CHARACTERISTICS:
  • Histologically confirmed cutaneous melanoma\*
  • Stage IV disease (i.e., distant metastasis)
  • Not curable by surgical resection
  • NOTE: \*Metastatic melanoma with an unknown primary tumor allowed provided ocular melanoma can be definitely excluded and origin from the skin is likely
  • Unidimensionally or bidimensionally measurable disease by physical examination and/or noninvasive radiological procedures
  • At least 1 measurable metastasis that has not been previously excised or biopsied
  • Failed ≥ 1 standard chemotherapy or chemoimmunotherapy regimen (e.g., dacarbazine or cisplatin monotherapy)
  • At least 1 metastatic lesion surgically accessible\* for excision or biopsy to obtain tumor material for RNA isolation\*\* NOTE: \*Surgically accessible metastatic lesion not required provided properly processed tumor material or isolated tumor RNA is available from a metastasis excised or biopsied within the past 6 months
  • NOTE: \*\*Major surgery not allowed for the acquisition of metastatic material solely for RNA isolation
  • No active CNS metastases by CT scan or MRI
  • Previously treated CNS metastases (e.g., by excision of a single metastasis, gamma knife radiosurgery, or stereotactic radiotherapy) allowed provided there is no evidence of active CNS metastasis by CT scan or MRI
  • PATIENT CHARACTERISTICS:
  • Age
  • Over 18
  • Performance status
  • Karnofsky 60-100%
  • Life expectancy
  • At least 4 months
  • Hematopoietic
  • WBC \> 2,500/mm\^3
  • Neutrophil count \> 1,000/mm\^3
  • Lymphocyte count \> 700/mm\^3
  • Platelet count \> 75,000/mm\^3
  • Hemoglobin \> 9 g/dL
  • No bleeding disorders
  • Hepatic
  • Bilirubin \< 2.0 mg/dL
  • Hepatitis B surface antigen negative
  • Hepatitis C antibody negative
  • Renal
  • Creatinine \< 2.5 mg/dL
  • Cardiovascular
  • No clinically significant heart disease
  • Pulmonary
  • No respiratory disease
  • Immunologic
  • HIV-1 or -2 negative
  • Human T-cell lymphotropic virus type I negative
  • No known hypersensitivity to dimethylsulfoxide
  • No immunodeficiency disease
  • No active systemic infection
  • No active autoimmune disease (except vitiligo), including any of the following:
  • Lupus erythematosus
  • Scleroderma
  • Rheumatoid arthritis (i.e., rheumatoid factor-positive arthritis with current or recent flare)
  • Ankylosing spondylitis
  • Autoimmune thyroiditis or uveitis
  • Autoimmune hemolytic anemia
  • Immune thrombocytopenic purpura
  • Multiple sclerosis
  • Inflammatory bowel disease
  • Other
  • Not pregnant or nursing
  • Negative pregnancy test
  • Fertile female patients must use effective contraception during and for ≥ 4 weeks after completion of study treatment
  • Willing to undergo excision or biopsy of metastasis
  • Willing to be hospitalized for ≥ 24 hours after each vaccination
  • Medical condition stable
  • No contraindication to leukapheresis
  • No organic brain syndrome
  • No significant psychiatric abnormality that would preclude study participation
  • No other active malignancy within the past 5 years except nonmelanoma skin cancer or carcinoma in situ of the cervix
  • No other major serious illness
  • PRIOR CONCURRENT THERAPY:
  • Biologic therapy
  • See Disease Characteristics
  • More than 4 weeks since prior systemic immunotherapy
  • No systemic immunotherapy during and for 2 weeks after completion of study treatment
  • Chemotherapy
  • See Disease Characteristics
  • More than 4 weeks since prior systemic chemotherapy
  • No systemic chemotherapy during and for 2 weeks after completion of study treatment
  • Endocrine therapy
  • No systemic corticosteroids, including steroid-containing inhalers or chronic use of topical steroids over large areas of the body (if systemic effects are likely or obvious) during and for 2 weeks after completion of study treatment
  • Radiotherapy
  • See Disease Characteristics
  • More than 2 weeks since prior radiotherapy
  • No prior radiotherapy to the spleen
  • Concurrent palliative radiotherapy to selected metastases for pain or local complications (e.g., compression) allowed
  • Surgery
  • See Disease Characteristics
  • Recovered from prior surgery
  • No prior splenectomy
  • No prior organ allograft
  • Concurrent palliative surgery to selected metastases for pain or local complications (e.g., compression) allowed
  • Other
  • Concurrent palliative hyperthermic therapy to selected metastases for pain or local complications (e.g., compression) allowed
  • No concurrent participation in any other clinical trial
  • No other systemic immunosuppressive agents (e.g., azathioprine or cyclosporine) during and for 2 weeks after completion of study treatment
  • No other investigational drugs or paramedical substances during and for 2 weeks after completion of study treatment

Exclusion

    Key Trial Info

    Start Date :

    April 1 2005

    Trial Type :

    INTERVENTIONAL

    Allocation :

    ESTIMATED

    End Date :

    Estimated Enrollment :

    30 Patients enrolled

    Trial Details

    Trial ID

    NCT00126685

    Start Date

    April 1 2005

    Last Update

    September 17 2013

    Active Locations (1)

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    Dermatologische Klinik mit Poliklinik - Universitaetsklinikum Erlangen

    Erlangen, Germany, D-91052