Status:

RECRUITING

Immunotherapy With Autologous Tumor Lysate-Loaded Dendritic Cells In Patients With Newly Diagnosed Glioblastoma Multiforme

Lead Sponsor:

Fondazione I.R.C.C.S. Istituto Neurologico Carlo Besta

Conditions:

Glioblastoma

Eligibility:

All Genders

18-70 years

Phase:

PHASE1

PHASE2

Brief Summary

Rationale of the Study: Treatment for GBM currently consists of surgical resection of the tumour mass followed by radio- and chemotherapy ((1)Stupp et al., 2005). Nonetheless overall prognosis still r...

Detailed Description

Background and significance. The therapeutic program will include radical surgical resection of the tumor, followed by radiotherapy (fractionated local field irradiation in daily fractions of 2 Gy giv...

Eligibility Criteria

Inclusion

  • Age ≥18 years and ≤70 years.
  • Postoperative Karnofsky Performance Status ≥70.
  • First diagnosis of GBM (World Health Organization \[WHO\] grade IV astrocytoma).
  • Diagnosis confirmed by the reference histopathology.
  • Residual tumor volume after resection \<10 cc, confirmed by postoperative MRI assessment
  • Total or subtotal resection of tumor mass, confirmed by assessment of the neurosurgeon and by postoperative radiological assessment.
  • Amount of non-necrotic tissue for lysate preparation and DC loading ≥1 gr, stored at -80°C.
  • Corticosteroids daily dose ≤4 mg during the 2 days prior to leukapheresis.
  • Clinical indication for radiochemotherapy according to the Stupp protocol (Stupp et al., 2005).
  • Life expectancy \> 3 months.
  • Informed consent

Exclusion

  • Pregnancy.
  • Participation in other clinical trials with experimental drugs simultaneously or within 1 month before this trial entry.
  • Presence of acute infection requiring active treatment.
  • Mandatory treatment with corticosteroids or salicylates in anti-inflammatory dose.
  • Presence of sub-ependymal diffusion of the tumor.
  • Presence of multi-focal GBM lesions.
  • Haematology: leukocytes \< 3,000/μl, lymphocytes \< 500/μl, neutrophils \< 1,000/μl, hemoglobin \<9 g/100 ml, thrombocytes \< 100,000/μl one or two days prior to leukapheresis.
  • Documented immune deficiency.
  • Documented autoimmune disease.
  • Positive serology for HIV, HBs antigen, HCV, TPHA.
  • Allergies to any component of the DC vaccine.
  • Known intolerance to TMZ.
  • Other active malignancy.

Key Trial Info

Start Date :

June 26 2010

Trial Type :

INTERVENTIONAL

Allocation :

ESTIMATED

End Date :

December 1 2024

Estimated Enrollment :

76 Patients enrolled

Trial Details

Trial ID

NCT04801147

Start Date

June 26 2010

End Date

December 1 2024

Last Update

November 8 2024

Active Locations (1)

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1

UOC Neuro-oncologia Molecolare

Milan, Italy, 20133