Status:

RECRUITING

Phase 1/2 Study of CD19 Chimeric Antigen Receptor T-cell (CD19 CAR-T; PL001) for Relapsed or Refractory B-cell Lymphoma

Lead Sponsor:

Pell Bio-Med Technology Co., Ltd.

Conditions:

Diffuse Large B Cell Lymphoma

Primary Mediastinal Large B Cell Lymphoma

Eligibility:

All Genders

14+ years

Phase:

PHASE1

PHASE2

Brief Summary

This is a multiple center, non-randomized, open-label, phase 1/2 study. The primary objective of Phase 1 is to evaluate the safety of PL001 and find the recommended Phase 2 dose (RP2D). The objective ...

Detailed Description

Cluster of differentiation (CD) 19 chimeric antigen receptor T-cell (CAR-T) has been a very promising treatment option for multiple types of B-cell lymphoma. Kymriah® (tisagenlecleucel, Novartis) and ...

Eligibility Criteria

Inclusion

  • Screening 1:
  • Patient is ≥14 years of age, inclusive, at the time of signing the informed consent.
  • Histologically confirmed diagnosis of diffuse large B-cell lymphoma (DLBCL), primary mediastinal large B-cell lymphoma (PMLBCL), large B-cell lymphoma transformed from follicular lymphoma (FL), or grade 3a or 3b FL.
  • On-site documentation of CD19 on the dominant population of cancer cells.
  • Disease status should meet any one of the below:
  • Patients with previous autologous-hematopoietic stem cell transplantation (auto HSCT) have relapsed, progressive, or refractory disease (defined as having not achieved a CR) after transplantation regardless of lines of systemic therapy.
  • Patients without previous HSCT have relapsed, progressive, or refractory disease (defined as having not achieved a CR) after at least 2 lines of systemic therapy, including anti-CD20 antibody and anthracycline.
  • Have no available effective systemic therapy as judged by the Investigator.
  • At least one measurable non-CNS (central nervous system) lesion based on Lugano classification for lymphoma.
  • Eastern Cooperative Oncology Group (ECOG) performance status of 0 to 2.
  • Life expectancy of at least 3 months.
  • Patient is male or female.
  • A male patient must agree to use a highly effective contraception as detailed in Section 10.4 (Appendix 4) during the treatment period and for at least 2 years after the dose of PL001 and refrain from donating sperm during this period.
  • Female Patients:
  • A female patient is eligible to participate if she is not pregnant (Section 10.4; Appendix 4), not breastfeeding, and at least one of the following conditions applies:
  • • Not a woman of childbearing potential (WOCBP) as defined in Section 10.4 (Appendix 4).
  • OR
  • • A WOCBP who agrees to follow the contraceptive guidance in Section 10.4 (Appendix 4) during the treatment period and for at least 2 years after the dose of PL001 and refrain from donating ova during this period.
  • Patient/patient's parent/legal guardian is capable of giving signed informed consent which includes compliance with the requirements and restrictions listed in the informed consent form (ICF) and in this protocol.
  • Screening 2:
  • Eastern Cooperative Oncology Group (ECOG) performance status of 0 to 2.
  • CAR-T is successfully manufactured and ready for use, from cells harvested by non mobilized leukapheresis.
  • WOCBP who have a negative serum pregnancy test at Screening 2.

Exclusion

  • Screening 1:
  • Chronic lymphocytic leukemia with Richter's transformation.
  • Primary CNS lymphoma. (Non-primary CNS lymphoma with CNS involvement is eligible).
  • Primary intra-ocular lymphoma.
  • Prior CD19 targeted therapy, such as CAR-T, Bi-specific T-cell engagers (BiTE), or monoclonal antibody.
  • History of cancers (includes myelodysplastic syndrome) other than non-melanoma skin cancer or carcinoma in situ (e.g., cervix, bladder, breast) unless disease-free without active treatment for at least 3 years.
  • History of allogeneic HSCT.
  • History of autologous HSCT within 3 months prior to consent.
  • Received any investigational product within 4 weeks prior to consent.
  • Systemic anticancer therapy within 3 weeks prior to apheresis.
  • Long-term use of systemic corticosteroids, defined as daily use \>10 mg of prednisolone or equivalent, within 2 weeks prior to leukapheresis.
  • Exception examples:
  • Nasal, ophthalmic, inhaled, intra-articular, and topical steroid preparation.
  • Short term systemic steroid for drug, contrast, or blood transfusion allergic reaction management.
  • Low dose maintenance steroid therapy for other conditions (e.g., asthma).
  • Use of long-acting and short-acting myeloid growth factor within 2 weeks, 5 days prior to leukapheresis, respectively.
  • Received anti-thymocyte globulin within 4 weeks prior to consent.
  • Intrathecal chemotherapy within 1 week prior to leukapheresis.
  • Inadequate major organ functions at Screening, which were defined as any of below:
  • absolute neutrophil count (ANC) \<500/µL
  • Absolute lymphocyte count (ALC) \<300/µL, excluding leukemic cells.
  • Hemoglobin (Hb) \<8.0 g/dL
  • Platelet count \<75,000/µL without transfusion support within 3 days
  • e. Baseline O2 saturation \<92% by pulse oximetry at room air
  • Significant CNS diseases such as dementia, major stroke, seizure while under antiepileptic drug
  • Aspartate aminotransferase (AST) \>5 × upper limit of normal (ULN) and alanine aminotransferase (ALT) \>5 × ULN, or total bilirubin \>2 × ULN (except for constitutional jaundice)
  • Serum creatinine \> 1.5 × ULN and estimated glomerular filtration rate (eGFR) \< 60 mL/min/1.73 m², as calculated by the Cockcroft-Gault formula.
  • Significant cardiac disease including but not limited to: left ventricular ejection fraction (LVEF) \<50%, QTc(the corrected QT interval) \> 480 msec based on Fredericia's formula, clinically significant arrhythmias, history of myocardial infarction or unstable angina within 3 months prior to consent.
  • Active hepatitis B virus (HBV) infection defined as detectable HBV DNA (Patients with positive anti-hepatitis B core antibody \[HBcAb\] must consent to regular monitoring of HBV DNA, and anti-HBV prophylaxis with oral anti-viral agent (such as entecavir) is mandatory until End-of-Study visit \[Visit 15\].)
  • Active hepatitis C virus (HCV) infection defined as positive anti- HCV antibody plus detectable HCV RNA.
  • Positive for human immunodeficiency virus (HIV) or human T-cell lymphotropic virus (HTLV) infection.
  • Uncontrolled acute life-threatening bacterial, viral, or fungal infection (e.g., the need for intravenous therapeutic antibiotics, blood culture positive ≤72 hours prior to apheresis).
  • 21\. Any medical conditions which might compromise the patient's safety from leukapheresis, lymphodepletion chemotherapy, or CAR-T therapy and anticipated AEs, according to the Investigator's evaluation.
  • Patients with insufficient leukapheresis cells.
  • Screening 2:
  • Inadequate major organ functions at Screening which were defined as any of below:
  • ANC \<500/µL
  • Hb \<8.0 g/dL
  • Platelet count \<50,000/µL, without transfusion support within 3 days
  • Baseline O2 saturation \<92% by pulse oximetry on room air
  • AST \>5 × ULN and ALT\>5 × ULN, or total bilirubin \>2 × ULN (except for constitutional jaundice)
  • Significant CNS diseases such as dementia, major stroke, seizure while under antiepileptic drug
  • Serum creatinine \> 1.5 × ULN and estimated glomerular filtration rate (eGFR) \< 60 mL/min/1.73 m², as calculated by the Cockcroft-Gault formula.
  • Long-term use of systemic corticosteroids, defined as daily use \>10 mg of prednisolone or equivalent.
  • Exception examples:
  • Nasal, ophthalmic, inhaled, intra-articular, and topical steroid preparation.
  • Short term systemic steroid for drug, contrast, or blood transfusion allergic reaction management.
  • Low dose maintenance steroid therapy for other conditions (e.g., asthma).
  • Use of long-acting and short-acting myeloid growth factor within 12 days, 2 days prior to lymphodepletion therapy, respectively.
  • Uncontrolled acute life-threatening bacterial, viral, or fungal infection (e.g. the need for intravenous therapeutic antibiotics, blood culture positive ≤72 hours prior to lymphodepletion).
  • Any medical condition which might compromise the patient's safety because of lymphodepletion chemotherapy or CAR-T therapy and anticipated AEs, according to the Investigator's opinion.

Key Trial Info

Start Date :

May 31 2022

Trial Type :

INTERVENTIONAL

Allocation :

ESTIMATED

End Date :

March 31 2027

Estimated Enrollment :

49 Patients enrolled

Trial Details

Trial ID

NCT05326243

Start Date

May 31 2022

End Date

March 31 2027

Last Update

May 13 2025

Active Locations (5)

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Page 1 of 2 (5 locations)

1

National Taiwan University Hospital

Taipei, Taiwan, Taiwan, 100225

2

Kaohsiung Medical University Chung-Ho Memorial Hospital

Kaohsiung City, Taiwan, 807377

3

Chi Mei Medical Center

Tainan, Taiwan, 710

4

Taipei Medical University - Taipei Medical University Hospital

Taipei, Taiwan, 11031

Phase 1/2 Study of CD19 Chimeric Antigen Receptor T-cell (CD19 CAR-T; PL001) for Relapsed or Refractory B-cell Lymphoma | DecenTrialz