Status:

RECRUITING

A Study of WZTL-002 CAR T-cells for Adults With Relapsed Large B-cell Lymphoma

Lead Sponsor:

Malaghan Institute of Medical Research

Collaborating Sponsors:

BioOra Limited

Wellington Zhaotai Therapies Limited

Conditions:

Large B-cell Lymphoma

Diffuse Large B-Cell Lymphoma, Not Otherwise Specified

Eligibility:

All Genders

18-75 years

Phase:

PHASE2

Brief Summary

The goal of this clinical trial is to learn if a new type of chimeric antigen receptor (CAR) T-cell therapy called WZTL-002 is effective and safe for the treatment large B-cell lymphomas (LBCL) that h...

Detailed Description

WZTL-002 is a third-generation CAR T-cell product comprising autologous T-cells transduced to express a CAR directed against CD19 and incorporating a Toll-like receptor 2 (TLR2) co-stimulatory domain ...

Eligibility Criteria

Inclusion

  • Age 18 to 75 years (inclusive) at the time of informed consent
  • Signed written informed consent for this trial
  • Biopsy-proven relapsed or treatment-refractory B-cell non-Hodgkin lymphoma of the following subtypes, as per the 2022 WHO classification of haematolymphoid tumours
  • Large B-cell lymphomas of the following histological subtypes:
  • Diffuse LBCL, not otherwise specified
  • Diffuse large B-cell lymphoma/high grade B-cell lymphoma with MYC and BCL2 rearrangements
  • Large B-cell lymphoma with IRF4 rearrangement
  • High grade B-cell lymphoma with 11q aberrations
  • High grade B-cell lymphoma, not otherwise specified
  • Primary mediastinal large B-cell lymphoma
  • Follicular large B-cell lymphoma
  • EBV-positive diffuse large B-cell lymphoma, not otherwise specified
  • Diffuse large B-cell lymphoma associated with chronic inflammation
  • Primary cutaneous DLBCL, leg type
  • Large B-cell lymphoma of one of the above subtypes that has transformed from follicular or marginal zone lymphoma
  • Received adequate first-line lymphoma therapy for the qualifying histology (as defined in inclusion criterion 3 above), comprising at least 2 cycles of a standard combination regimen incorporating an anthracycline and an anti-CD20 monoclonal antibody
  • Relapsed or refractory disease meeting one of the following criteria:
  • Relapsed or refractory within 12 months of first-line chemoimmunotherapy, defined as:
  • Progressive disease following ≥ 2 cycles of chemoimmunotherapy, or
  • Stable disease following ≥ 4 cycles of chemoimmunotherapy, or
  • Partial response following ≥ 6 cycles of chemoimmunotherapy, or
  • Complete response followed by biopsy-proven relapse within 12 months of completing first-line chemoimmunotherapy.
  • Relapsed or refractory following second-line chemoimmunotherapy, defined as:
  • Lack of complete response to, or relapse following, autologous stem cell transplantation as part of second-line therapy for the qualifying histology, or
  • Inability to proceed to autologous stem cell transplantation due to lack of response to 2 cycles of second-line chemoimmunotherapy incorporating both a platinum agent and an anti-CD20 monoclonal antibody
  • Positron emission tomography (PET) positive disease according to the Lugano 2014 criteria
  • Available tumour tissue (comprising a tissue block or at least 6 unstained slides) for central histological review
  • Lymphoma-related life expectancy at least 12 weeks, and life expectancy related to conditions other than lymphoma at least 12 months
  • ECOG performance status of 0 or 1
  • Adequate haematologic function, defined by:
  • Neutrophils ≥ 1.0 × 10\^9/L, and Platelets ≥ 75 × 10\^9/L, and
  • Lymphocytes ≥ 0.3 × 10\^9/L
  • Adequate renal function, defined by estimated creatinine clearance (eCrCl) or glomerular filtration rate (eGFR) \>/= 45mL/min using the Cockroft Gault estimation, CKD-EPI equation or as assessed by direct measurement.
  • Adequate hepatic function, defined by serum bilirubin \< 2.5 × upper limit of normal (ULN) (unless attributable to Gilbert's syndrome) and alanine transaminase and aspartate aminotransferase \< 3 × ULN.
  • Adequate lung function, defined as ≤ Grade 1 dyspnoea according to NCI CTCAE v5.0, and oxygen saturation (sO2) ≥ 92% on room air.
  • Adequate cardiac function, defined as left ventricular ejection fraction (LVEF) ≥ 40% as assessed by echocardiogram or multigated acquisition (MUGA), performed within 28 days of commencing screening.
  • For female participants:
  • Agree to use a condom if undertaking sexual activity with any partner during lymphodepleting chemotherapy, and
  • If of reproductive potential agree to use a highly effective method of contraception from the time of enrolment until at least 12 months after administration of WZTL-002, or
  • Are not of reproductive potential defined as either,
  • being amenorrhoeic for at least 12 consecutive months with FSH 30 ≥ IU/L, or
  • previously undergone a sterilisation procedure
  • For male participants:
  • Agree to use a condom if undertaking sexual activity with any partner during lymphodepleting chemotherapy, and
  • If undertaking sexual activity with a female partner of reproductive potential agree to use a highly effective method of contraception from the time of enrolment until at least 12 months after administration of WZTL-002, and
  • Agree not to donate sperm for conception, or to provide gametes for in vitro fertilisation for at least 12 months after administration of WZTL-002
  • Participant agrees not to donate blood components at any time after receiving WZTL-002

Exclusion

  • Active central nervous system (CNS) involvement by lymphoma. In patients with a history of CNS disease or a clinical suspicion of current CNS disease, lumbar puncture and MRI brain must be performed within 30 days of enrolment to exclude current CNS involvement.
  • Active CNS pathology including: epilepsy, seizure within the preceding year, aphasia, paresis, stroke, dementia, psychosis within the preceding year, severe brain injury, Parkinson disease, or cerebellar disease
  • B-cell non-Hodgkin lymphoma of the following subtypes, as per the 2022 WHO classification of haematolymphoid tumours:
  • Richter transformation of chronic lymphocytic leukaemia
  • T-cell/histiocyte rich LBCL
  • Primary LBCL of immune-privileged sites
  • Fluid overload associated LBCL
  • Fibrin-associated LBCL
  • Plasmablastic lymphoma
  • Mediastinal grey zone lymphoma
  • Intravascular LBCL
  • ALK-positive large B-cell lymphoma
  • Lymphomatoid granulomatosis
  • Burkitt lymphoma
  • Primary effusion lymphoma
  • KSHV/HHV8-positive diffuse large B-cell lymphoma
  • Patient has received 3 or more prior lines of therapy for LBCL, where 1 line of therapy is defined as 1 or more cycles of a combination chemoimmunotherapy with or without pre-planned consolidation therapy (radiotherapy, autologous stem cell transplant or immunotherapy)
  • Requirement for urgent lymphoma therapy due to tumour-related symptoms, or due to imminent risk of blood vessel, airway, urinary tract, gastrointestinal tract, nerve or spinal cord compression
  • Active autoimmune disease requiring current systemic immunosuppression
  • Active sarcoidosis
  • Prior solid organ transplantation or prior allogeneic stem cell transplantation (allo-SCT)
  • Peripheral blood CD3+ T cells \< 150/μL (0.15 x10\^9/L) as assessed by lymphocyte subset analysis
  • History of active malignancy other than B-cell malignancy within 2 years prior to enrolment, with the exception of: adequately treated in situ carcinoma of the cervix; adequately treated basal cell carcinoma (BCC) or squamous cell carcinoma (SCC) of the skin; other localised malignancy surgically resected (or radically treated with another treatment modality) with curative intent
  • Prior treatment with:
  • gene therapy (including CAR T-cell therapy) or CD19-targeted immunotherapy, or
  • purine analogue (including bendamustine) or alemtuzumab within 6 months of enrolment, or
  • bispecific T-cell engager, radiotherapy or an investigational medicine within 4 weeks of enrolment, or
  • cytotoxic chemotherapy, systemic corticosteroids (at doses of ≥ 10 mg prednisone daily or equivalent), monoclonal antibody or antibody-drug conjugate (other than alemtuzumab) within 2 weeks of enrolment.
  • Pregnant or lactating female
  • Known sensitivity to immunoglobulin or to components of the IP
  • Current or prior HIV infection
  • Vaccination with a live virus within the 4 weeks of enrolment
  • Inadequately-controlled systemic infection
  • Serologic status reflecting active viral hepatitis B or active hepatitis C infection as follows:
  • Presence of hepatitis B surface antigen (HBsAg) or hepatitis B core antibody (HBcAb). Patients with presence of HBcAb, but absence of HBsAg, are eligible if hepatitis B virus (HBV) DNA is undetectable (or is \< 20 IU/mL), and if they are willing to receive appropriate antiviral prophylaxis.
  • Presence of active Hepatitis C infection as determined by Hepatitis C virus (HCV) RNA detected by PCR or nucleic acid testing (NAT). Patients with presence of HCV antibody, are eligible if HCV RNA is undetectable.
  • Current New York Heart Association (NYHA) class 2 or higher cardiac symptoms, or myocardial infarction, unstable angina or other clinically significant cardiac disease within the past 6 months
  • Significant concomitant illnesses which would in the Investigators opinion make the patient an unsuitable candidate for the trial
  • Patients who have diminished capacity or any circumstance that would prohibit them from understanding and providing informed consent in accordance with ICH-GCP
  • Patient does not provide consent to enrol to an International Cellular Therapy Registry

Key Trial Info

Start Date :

July 12 2024

Trial Type :

INTERVENTIONAL

Allocation :

ESTIMATED

End Date :

June 30 2028

Estimated Enrollment :

60 Patients enrolled

Trial Details

Trial ID

NCT06486051

Start Date

July 12 2024

End Date

June 30 2028

Last Update

February 14 2025

Active Locations (3)

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Page 1 of 1 (3 locations)

1

Christchurch Hospital

Christchurch, Christchurch Central, New Zealand, 8011

2

Wellington Hospital

Newtown, Wellington Region, New Zealand, 6021

3

Auckland City Hospital

Auckland, New Zealand, 1023