Status:

RECRUITING

Effect of Agricultural Practices on Crops, Gut Microbiome, and Human Health

Lead Sponsor:

Amsterdam University Medical Centers (UMC), Location Academic Medical Center (AMC)

Conditions:

Obesity Prevention

Microbiome Analysis

Eligibility:

All Genders

18-45 years

Phase:

NA

Brief Summary

The human gut microbiome plays a regulatory role in host health, and is involved in metabolic, immune, and neurological processes. Diet shapes the gut microbiome; by providing essential nutrients, whi...

Detailed Description

Objective: The primary objective of this study is to investigate the effect of differentially cultivated crops (organic versus conventional) on cardiometabolic health outcomes, as reflected by measur...

Eligibility Criteria

Inclusion

  • 22 Healthy Volunteers : healthy Caucasian adults with a BMI \< 25 kg/m\^2 will be recruited with no medical conditions.
  • 22 Metabolically Impaired Participants: Caucasian adults with a BMI ranging from 28 kg/m\^2 to 40 kg/m\^2 with three components of Metabolic Syndrome, drug-naïve:
  • Elevated waist circumference (WC): with men having WC \> 102 cm (40 inches) and women having a WC \> 88 cm (35 inches).
  • High Fasting Plasma Glucose (FPG): a fasting plasma glucose level 110 mg/dL (6.1 mmol/L)
  • High Fasting Plasma Insulin (FPI): A fasting insulin level ≥25 μU/mL (or \>16.7 pmol/L)
  • HOMA-IR score of ≥2.5

Exclusion

  • Use of antibiotic in the past 3 months or currently taking them
  • Past/ Currently following a vegan, strictly organic or vegetarian diet.
  • History of severe food allergies or intolerances that would render it unsafe for them to consume any of the fruits and vegetables provided. Exclusion will be determined on a case-by-case basis by the investigator.
  • Inability or unwillingness to collect stools.
  • Presence of overt type 1 diabetes mellitus (T1DM) and/or type 2 diabetes.
  • Use of medications for type 2 diabetes: metformin, statin, proton pump inhibitors (PPI), H2 blockers, pioglitazone, active GLP-1 receptor agonists (e.g., exenatide, liraglutide).
  • Prior or planned bariatric surgery, history of cholecystectomy.
  • History of cardiovascular disease, having a severe disease of the digestive tract, such as celiac disease, Crohn's disease, active ulcerative colitis.
  • Surgery scheduled for the trial duration period.
  • Pregnant or nursing women.
  • Smoking or illicit drug use. Use of \>5 units of alcohol daily on average.
  • History of neurological/neurodegenerative disorders.
  • Participation in another concomitant clinical trial.
  • Not residing in the Netherlands during study period (Sept-Nov 2025)

Key Trial Info

Start Date :

June 25 2025

Trial Type :

INTERVENTIONAL

Allocation :

ESTIMATED

End Date :

July 5 2027

Estimated Enrollment :

44 Patients enrolled

Trial Details

Trial ID

NCT07165145

Start Date

June 25 2025

End Date

July 5 2027

Last Update

September 10 2025

Active Locations (1)

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1

Amsterdam UMC, locatie AMC

Amsterdam, Amsterdam, Netherlands, 1105 AZ